7LWA

Human Exonuclease 5 crystal structure (T88E) in complex with ssDNA and Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1158
Spacegroup name	P 2 21 21
Unit cell lengths	50.512, 84.722, 95.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.517 - 2.850
R-factor	0.2058
Rwork	0.203
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lw7
RMSD bond length	0.002
RMSD bond angle	0.431
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.520	47.520	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge	0.362	0.062	2.352
Rmeas	0.377	0.065	2.443
Rpim	0.103	0.019	0.655
Number of reflections	9998	376	1423
<I/σ(I)>	8.1	27.6	1.4
Completeness [%]	99.7	99.7	99.6
Redundancy	13	10.9	13.5
CC(1/2)	0.992	0.999	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350 and 0.1 M magnesium formate