7JUK
Crystal structure of PTEN with a tetra-phosphorylated tail (4p-crPTEN-13sp-T2, SDTTDSDPENEG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2017-11-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | I 4 |
Unit cell lengths | 113.444, 113.444, 57.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.520 - 3.150 |
R-factor | 0.1887 |
Rwork | 0.184 |
R-free | 0.28890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1d5r |
RMSD bond length | 0.005 |
RMSD bond angle | 1.408 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 80.217 | 28.503 | 3.320 |
High resolution limit [Å] | 3.150 | 9.960 | 3.150 |
Rmerge | 0.086 | 0.715 | |
Rmeas | 0.152 | 0.093 | 0.779 |
Rpim | 0.059 | 0.035 | 0.306 |
Total number of observations | 41598 | 1424 | 5502 |
Number of reflections | 6346 | 213 | 878 |
<I/σ(I)> | 8.8 | 19.7 | 2.7 |
Completeness [%] | 99.3 | 96.6 | 95.9 |
Redundancy | 6.6 | 6.7 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 1.2 M DL-malic acid |