7BRE
The crystal structure of MLL2 in complex with ASH2L and RBBP5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.075, 66.195, 83.734 |
Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.159 | 0.081 | 0.642 |
Rmeas | 0.173 | 0.088 | 0.700 |
Rpim | 0.066 | 0.034 | 0.276 |
Total number of observations | 142941 | ||
Number of reflections | 21457 | 2216 | 2120 |
<I/σ(I)> | 3.4 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 6.7 | 6.4 | 6.3 |
CC(1/2) | 0.997 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Ammonium tartrate dibasic pH 7.0, 12%(w/v) Polyethylene glycol 3,350 |