6ZHC

PROTAC6 mediated complex of VHL:EloB:EloC and Bcl-xL

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	70.990, 101.180, 106.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.270 - 1.920
Rwork	0.169
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lm8 1maz
RMSD bond length	0.004
RMSD bond angle	1.252
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.270	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.079	0.638
Number of reflections	59379	3945
<I/σ(I)>	15.1	2.5
Completeness [%]	99.9	99.6
Redundancy	6.5	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	291	0.2 M Potassium iodide, 0.1 M MES pH 6.5, 23% PEG 4000