6Z88
human GTP cyclohydrolase I in complex with allosteric inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999859 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.355, 161.505, 271.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 135.820 - 2.687 |
| R-factor | 0.2189 |
| Rwork | 0.217 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fb1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.821 | 2.971 |
| High resolution limit [Å] | 2.687 | 2.687 |
| Number of reflections | 47926 | 2396 |
| <I/σ(I)> | 8.9 | 1.5 |
| Completeness [%] | 93.9 | 58.2 |
| Redundancy | 10.1 | 8.5 |
| CC(1/2) | 0.998 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.2 M Magnesium chloride, 10 % PEG 8000; TRIS pH 7.0 |
