6WGH

Crystal structure of GDP-bound NRAS with ten residues long internal tandem duplication in the switch II region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97911
Spacegroup name	P 1 21 1
Unit cell lengths	40.460, 45.960, 89.080
Unit cell angles	90.00, 99.77, 90.00

Refinement procedure

Resolution	43.890 - 1.650
R-factor	0.1793
Rwork	0.178
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3con
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.890	43.890	1.690
High resolution limit [Å]	1.650	7.380	1.650
Rmerge	0.099	0.052	0.787
Rmeas	0.108	0.056	0.857
Total number of observations	254986
Number of reflections	38763	482	2863
<I/σ(I)>	11.24	23.99	2.37
Completeness [%]	99.2	99.8	99
Redundancy	6.578	6.471	6.567
CC(1/2)	0.997	0.998	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M Na Citrate pH 5.0, 15% PEG 4000 and 0.1 M MgCl2