6VAX
Crystal structure of human SDHA-SDHAF2 assembly intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.558, 68.782, 126.578 |
| Unit cell angles | 90.00, 93.16, 90.00 |
Refinement procedure
| Resolution | 45.050 - 2.590 |
| R-factor | 0.2015 |
| Rwork | 0.199 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b58 1zoy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 56205 | 2855 |
| <I/σ(I)> | 24.7 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.991 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 100 mM Sodium malonate, 12 % w/v PEG 3350, 10 mM Oxaloacetate |
