1ZOY

Crystal Structure of Mitochondrial Respiratory Complex II from porcine heart at 2.4 Angstroms

Summary for 1ZOY

• Entry DOI: 10.2210/pdb1zoy/pdb
• Related: 1ZP0
• Descriptor: FAD-binding protein, PROTOPORPHYRIN IX CONTAINING FE, L-ALPHA-PHOSPHATIDYL-BETA-OLEOYL-GAMMA-PALMITOYL-PHOSPHATIDYLETHANOLAMINE, ... (12 entities in total)
• Functional Keywords: succinate, ubiquinone oxidoreductase, mitochondrial respiratory complex ii, membrane protein structure, oxidoreductase
• Biological source: Sus scrofa (pig); More
• Total number of polymer chains: 4
• Total formula weight: 127241.73

Authors

Sun, F.,Huo, X.,Zhai, Y.,Wang, A.,Xu, J.,Su, D.,Bartlam, M.,Rao, Z. (deposition date: 2005-05-15, release date: 2005-07-12, Last modification date: 2024-11-20)

Primary citation

Sun, F.,Huo, X.,Zhai, Y.,Wang, A.,Xu, J.,Su, D.,Bartlam, M.,Rao, Z.
Crystal Structure of Mitochondrial Respiratory Membrane Protein Complex II
Cell(Cambridge,Mass.), 121:1043-1057, 2005

PubMed Abstract: The mitochondrial respiratory Complex II or succinate:ubiquinone oxidoreductase (SQR) is an integral membrane protein complex in both the tricarboxylic acid cycle and aerobic respiration. Here we report the first crystal structure of Complex II from porcine heart at 2.4 A resolution and its complex structure with inhibitors 3-nitropropionate and 2-thenoyltrifluoroacetone (TTFA) at 3.5 A resolution. Complex II is comprised of two hydrophilic proteins, flavoprotein (Fp) and iron-sulfur protein (Ip), and two transmembrane proteins (CybL and CybS), as well as prosthetic groups required for electron transfer from succinate to ubiquinone. The structure correlates the protein environments around prosthetic groups with their unique midpoint redox potentials. Two ubiquinone binding sites are discussed and elucidated by TTFA binding. The Complex II structure provides a bona fide model for study of the mitochondrial respiratory system and human mitochondrial diseases related to mutations in this complex.

PubMed: 15989954
DOI: 10.1016/j.cell.2005.05.025

Experimental method

X-RAY DIFFRACTION (2.4 Å)