1ZOY
Crystal Structure of Mitochondrial Respiratory Complex II from porcine heart at 2.4 Angstroms
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.74101 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.238, 83.557, 293.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.232 |
Rwork | 0.213 |
R-free | 0.25900 |
Structure solution method | SAD combined with MR |
Starting model (for MR) | 1nek |
RMSD bond length | 0.018 |
RMSD bond angle | 1.810 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.125 | 0.501 |
Number of reflections | 60175 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 85.1 | 40.5 |
Redundancy | 9.1 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | n-nonyl-D-maltoside, n-decyl-D-maltoside, HEPES, PEG 4000, 1,6-hexanediol, NaCl, CaCl2, sucrose, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 290 | n-nonyl-D-maltoside, n-decyl-D-maltoside, HEPES, PEG 4000, 1,6-hexanediol, NaCl, CaCl2, sucrose, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |