1NEK

Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound

Summary for 1NEK

• Entry DOI: 10.2210/pdb1nek/pdb
• Related: 1nen
• Descriptor: Succinate dehydrogenase flavoprotein subunit, FE3-S4 CLUSTER, PROTOPORPHYRIN IX CONTAINING FE, ... (15 entities in total)
• Functional Keywords: membrane protein, oxygen respiratory chain, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• Biological source: Escherichia coli; More
• Cellular location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side: P0AC41; Cell inner membrane; Peripheral membrane protein: P07014; Cell inner membrane; Multi-pass membrane protein: P69054 P0AC44
• Total number of polymer chains: 4
• Total formula weight: 123107.58

Authors

Yankovskaya, V.,Horsefield, R.,Tornroth, S.,Luna-Chavez, C.,Miyoshi, H.,Leger, C.,Byrne, B.,Cecchini, G.,Iwata, S. (deposition date: 2002-12-11, release date: 2003-02-25, Last modification date: 2024-11-20)

Primary citation

Yankovskaya, V.,Horsefield, R.,Tornroth, S.,Luna-Chavez, C.,Miyoshi, H.,Leger, C.,Byrne, B.,Cecchini, G.,Iwata, S.
Architecture of succinate dehydrogenase and reactive oxygen species generation.
Science, 299:700-704, 2003

PubMed Abstract: The structure of Escherichia coli succinate dehydrogenase (SQR), analogous to the mitochondrial respiratory complex II, has been determined, revealing the electron transport pathway from the electron donor, succinate, to the terminal electron acceptor, ubiquinone. It was found that the SQR redox centers are arranged in a manner that aids the prevention of reactive oxygen species (ROS) formation at the flavin adenine dinucleotide. This is likely to be the main reason SQR is expressed during aerobic respiration rather than the related enzyme fumarate reductase, which produces high levels of ROS. Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme.

PubMed: 12560550
DOI: 10.1126/science.1079605

Experimental method

X-RAY DIFFRACTION (2.6 Å)