1NEK
Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0081 |
Spacegroup name | H 3 2 |
Unit cell lengths | 138.800, 138.800, 521.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.600 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.28900 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 53727 | |
Completeness [%] | 89.9 | 90 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | Tris-HCl, CaCl2, PEG 400, BaCl2, ethylene glycol, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |