6UE5
Crystal structure of full-length human DCAF15-DDB1-deltaPBP-DDA1-RBM39 in complex with 4-(aminomethyl)-N-(3-cyano-4-methyl-1H-indol-7-yl)benzenesulfonamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-17 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.692, 94.237, 267.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 78.130 - 2.610 |
R-factor | 0.241 |
Rwork | 0.239 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ud7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.790 | 2.690 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.487 | 3.221 |
Rmeas | 0.500 | 3.309 |
Rpim | 0.113 | 0.752 |
Number of reflections | 63346 | 4597 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.6 | 19.3 |
CC(1/2) | 0.985 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.5 | 2% (v:v) TacsimateTM, pH 5.0, 0.1 M sodium citrate tribasic 676 dihydrate, pH 5.6, and 10-20% (w:v) polyethylene glycol 3350 |