6SHT
Molecular structure of mouse apoferritin resolved at 2.7 Angstroms with the Glacios cryo-microscope
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.73 |
| Number of Particle | 211177 |
| Software | RELION,EPU,Gctf,UCSF Chimera,PHENIX |
| Symmetry | O |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
