- EMDB-4905: 3D structure of horse spleen apoferritin determined using multifu... -
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Basic information
Entry
Database: EMDB / ID: EMD-4905
Title
3D structure of horse spleen apoferritin determined using multifunctional graphene supports for electron cryomicroscopy
Map data
Post-processed, masked and filtered map
Sample
Complex: Apoferritin
Protein or peptide: Ferritin light chain
Keywords
METAL TRANSPORT
Function / homology
Function and homology information
: / intracellular sequestering of iron ion / ferric iron binding / ferrous iron binding / iron ion transport / iron ion binding / cytoplasm Similarity search - Function
Journal: Proc Natl Acad Sci U S A / Year: 2019 Title: Multifunctional graphene supports for electron cryomicroscopy. Authors: Katerina Naydenova / Mathew J Peet / Christopher J Russo / Abstract: With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In ...With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows high-resolution structure determination with a minimum of material and data.
History
Deposition
Apr 26, 2019
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Header (metadata) release
Jun 5, 2019
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Map release
Jun 5, 2019
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Update
May 22, 2024
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Current status
May 22, 2024
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
EMPIAR-10272 (Title: Movies of horse spleen apoferritin on multifunctional ultrastable graphene supports for electron cryomicroscopy Data size: 570.0 Data #1: Movies of apoferritin on functionalized ultrastable graphene support [micrographs - multiframe])
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