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- PDB-6rjh: 3D structure of horse spleen apoferritin determined using multifu... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rjh | ||||||
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Title | 3D structure of horse spleen apoferritin determined using multifunctional graphene supports for electron cryomicroscopy | ||||||
![]() | Ferritin light chain | ||||||
![]() | METAL TRANSPORT | ||||||
Function / homology | ![]() : / intracellular sequestering of iron ion / ferric iron binding / ferrous iron binding / iron ion transport / iron ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.1 Å | ||||||
![]() | Naydenova, K. / Peet, M.J. / Russo, C.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Multifunctional graphene supports for electron cryomicroscopy. Authors: Katerina Naydenova / Mathew J Peet / Christopher J Russo / ![]() Abstract: With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In ...With recent technological advances, the atomic resolution structure of any purified biomolecular complex can, in principle, be determined by single-particle electron cryomicroscopy (cryoEM). In practice, the primary barrier to structure determination is the preparation of a frozen specimen suitable for high-resolution imaging. To address this, we present a multifunctional specimen support for cryoEM, comprising large-crystal monolayer graphene suspended across the surface of an ultrastable gold specimen support. Using a low-energy plasma surface modification system, we tune the surface of this support to the specimen by patterning a range of covalent functionalizations across the graphene layer on a single grid. This support design reduces specimen movement during imaging, improves image quality, and allows high-resolution structure determination with a minimum of material and data. | ||||||
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Structure visualization
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Structure viewer | Molecule: ![]() ![]() |
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PDBx/mmCIF format | ![]() | 779.7 KB | Display | ![]() |
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PDB format | ![]() | 649.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 119.4 KB | Display | |
Data in CIF | ![]() | 150.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4905MC M: map data used to model this data C: citing same article ( |
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Similar structure data | |
EM raw data | ![]() Data size: 570.0 Data #1: Movies of apoferritin on functionalized ultrastable graphene support [micrographs - multiframe]) |
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Assembly
Deposited unit | ![]()
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Noncrystallographic symmetry (NCS) | NCS domain:
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