6OVG
L-Methionine Depletion with an Engineered Human Enzyme Disrupts Prostate Cancer Metabolism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03321 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 113.558, 164.330, 181.696 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.337 - 2.719 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nmp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.677 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.719 | 2.719 |
Number of reflections | 175177 | 5110 |
<I/σ(I)> | 13.109 | |
Completeness [%] | 99.4 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% isopropanol, 150mM sodium citrate 100mM sodium cocadylate pH 6.5 |