Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6OVG

L-Methionine Depletion with an Engineered Human Enzyme Disrupts Prostate Cancer Metabolism

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004123	molecular_function	cystathionine gamma-lyase activity
A	0005515	molecular_function	protein binding
A	0005516	molecular_function	calmodulin binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006534	biological_process	cysteine metabolic process
A	0006629	biological_process	lipid metabolic process
A	0016829	molecular_function	lyase activity
A	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
A	0019343	biological_process	cysteine biosynthetic process via cystathionine
A	0019344	biological_process	cysteine biosynthetic process
A	0019346	biological_process	transsulfuration
A	0030170	molecular_function	pyridoxal phosphate binding
A	0030968	biological_process	endoplasmic reticulum unfolded protein response
A	0042802	molecular_function	identical protein binding
A	0043066	biological_process	negative regulation of apoptotic process
A	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
A	0044524	biological_process	protein sulfhydration
A	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
A	0047982	molecular_function	homocysteine desulfhydrase activity
A	0051289	biological_process	protein homotetramerization
A	0070062	cellular_component	extracellular exosome
A	0070814	biological_process	hydrogen sulfide biosynthetic process
A	0080146	molecular_function	L-cysteine desulfhydrase activity
A	0098606	molecular_function	selenocystathionine gamma-lyase activity
A	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
A	1990830	biological_process	cellular response to leukemia inhibitory factor
A	2001234	biological_process	negative regulation of apoptotic signaling pathway
B	0004123	molecular_function	cystathionine gamma-lyase activity
B	0005515	molecular_function	protein binding
B	0005516	molecular_function	calmodulin binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006534	biological_process	cysteine metabolic process
B	0006629	biological_process	lipid metabolic process
B	0016829	molecular_function	lyase activity
B	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
B	0019343	biological_process	cysteine biosynthetic process via cystathionine
B	0019344	biological_process	cysteine biosynthetic process
B	0019346	biological_process	transsulfuration
B	0030170	molecular_function	pyridoxal phosphate binding
B	0030968	biological_process	endoplasmic reticulum unfolded protein response
B	0042802	molecular_function	identical protein binding
B	0043066	biological_process	negative regulation of apoptotic process
B	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
B	0044524	biological_process	protein sulfhydration
B	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
B	0047982	molecular_function	homocysteine desulfhydrase activity
B	0051289	biological_process	protein homotetramerization
B	0070062	cellular_component	extracellular exosome
B	0070814	biological_process	hydrogen sulfide biosynthetic process
B	0080146	molecular_function	L-cysteine desulfhydrase activity
B	0098606	molecular_function	selenocystathionine gamma-lyase activity
B	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
B	1990830	biological_process	cellular response to leukemia inhibitory factor
B	2001234	biological_process	negative regulation of apoptotic signaling pathway
C	0004123	molecular_function	cystathionine gamma-lyase activity
C	0005515	molecular_function	protein binding
C	0005516	molecular_function	calmodulin binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006534	biological_process	cysteine metabolic process
C	0006629	biological_process	lipid metabolic process
C	0016829	molecular_function	lyase activity
C	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
C	0019343	biological_process	cysteine biosynthetic process via cystathionine
C	0019344	biological_process	cysteine biosynthetic process
C	0019346	biological_process	transsulfuration
C	0030170	molecular_function	pyridoxal phosphate binding
C	0030968	biological_process	endoplasmic reticulum unfolded protein response
C	0042802	molecular_function	identical protein binding
C	0043066	biological_process	negative regulation of apoptotic process
C	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
C	0044524	biological_process	protein sulfhydration
C	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
C	0047982	molecular_function	homocysteine desulfhydrase activity
C	0051289	biological_process	protein homotetramerization
C	0070062	cellular_component	extracellular exosome
C	0070814	biological_process	hydrogen sulfide biosynthetic process
C	0080146	molecular_function	L-cysteine desulfhydrase activity
C	0098606	molecular_function	selenocystathionine gamma-lyase activity
C	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
C	1990830	biological_process	cellular response to leukemia inhibitory factor
C	2001234	biological_process	negative regulation of apoptotic signaling pathway
D	0004123	molecular_function	cystathionine gamma-lyase activity
D	0005515	molecular_function	protein binding
D	0005516	molecular_function	calmodulin binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006534	biological_process	cysteine metabolic process
D	0006629	biological_process	lipid metabolic process
D	0016829	molecular_function	lyase activity
D	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
D	0019343	biological_process	cysteine biosynthetic process via cystathionine
D	0019344	biological_process	cysteine biosynthetic process
D	0019346	biological_process	transsulfuration
D	0030170	molecular_function	pyridoxal phosphate binding
D	0030968	biological_process	endoplasmic reticulum unfolded protein response
D	0042802	molecular_function	identical protein binding
D	0043066	biological_process	negative regulation of apoptotic process
D	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
D	0044524	biological_process	protein sulfhydration
D	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
D	0047982	molecular_function	homocysteine desulfhydrase activity
D	0051289	biological_process	protein homotetramerization
D	0070062	cellular_component	extracellular exosome
D	0070814	biological_process	hydrogen sulfide biosynthetic process
D	0080146	molecular_function	L-cysteine desulfhydrase activity
D	0098606	molecular_function	selenocystathionine gamma-lyase activity
D	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
D	1990830	biological_process	cellular response to leukemia inhibitory factor
D	2001234	biological_process	negative regulation of apoptotic signaling pathway
E	0004123	molecular_function	cystathionine gamma-lyase activity
E	0005515	molecular_function	protein binding
E	0005516	molecular_function	calmodulin binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006534	biological_process	cysteine metabolic process
E	0006629	biological_process	lipid metabolic process
E	0016829	molecular_function	lyase activity
E	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
E	0019343	biological_process	cysteine biosynthetic process via cystathionine
E	0019344	biological_process	cysteine biosynthetic process
E	0019346	biological_process	transsulfuration
E	0030170	molecular_function	pyridoxal phosphate binding
E	0030968	biological_process	endoplasmic reticulum unfolded protein response
E	0042802	molecular_function	identical protein binding
E	0043066	biological_process	negative regulation of apoptotic process
E	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
E	0044524	biological_process	protein sulfhydration
E	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
E	0047982	molecular_function	homocysteine desulfhydrase activity
E	0051289	biological_process	protein homotetramerization
E	0070062	cellular_component	extracellular exosome
E	0070814	biological_process	hydrogen sulfide biosynthetic process
E	0080146	molecular_function	L-cysteine desulfhydrase activity
E	0098606	molecular_function	selenocystathionine gamma-lyase activity
E	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
E	1990830	biological_process	cellular response to leukemia inhibitory factor
E	2001234	biological_process	negative regulation of apoptotic signaling pathway
F	0004123	molecular_function	cystathionine gamma-lyase activity
F	0005515	molecular_function	protein binding
F	0005516	molecular_function	calmodulin binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006534	biological_process	cysteine metabolic process
F	0006629	biological_process	lipid metabolic process
F	0016829	molecular_function	lyase activity
F	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
F	0019343	biological_process	cysteine biosynthetic process via cystathionine
F	0019344	biological_process	cysteine biosynthetic process
F	0019346	biological_process	transsulfuration
F	0030170	molecular_function	pyridoxal phosphate binding
F	0030968	biological_process	endoplasmic reticulum unfolded protein response
F	0042802	molecular_function	identical protein binding
F	0043066	biological_process	negative regulation of apoptotic process
F	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
F	0044524	biological_process	protein sulfhydration
F	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
F	0047982	molecular_function	homocysteine desulfhydrase activity
F	0051289	biological_process	protein homotetramerization
F	0070062	cellular_component	extracellular exosome
F	0070814	biological_process	hydrogen sulfide biosynthetic process
F	0080146	molecular_function	L-cysteine desulfhydrase activity
F	0098606	molecular_function	selenocystathionine gamma-lyase activity
F	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
F	1990830	biological_process	cellular response to leukemia inhibitory factor
F	2001234	biological_process	negative regulation of apoptotic signaling pathway
G	0004123	molecular_function	cystathionine gamma-lyase activity
G	0005515	molecular_function	protein binding
G	0005516	molecular_function	calmodulin binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006534	biological_process	cysteine metabolic process
G	0006629	biological_process	lipid metabolic process
G	0016829	molecular_function	lyase activity
G	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
G	0019343	biological_process	cysteine biosynthetic process via cystathionine
G	0019344	biological_process	cysteine biosynthetic process
G	0019346	biological_process	transsulfuration
G	0030170	molecular_function	pyridoxal phosphate binding
G	0030968	biological_process	endoplasmic reticulum unfolded protein response
G	0042802	molecular_function	identical protein binding
G	0043066	biological_process	negative regulation of apoptotic process
G	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
G	0044524	biological_process	protein sulfhydration
G	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
G	0047982	molecular_function	homocysteine desulfhydrase activity
G	0051289	biological_process	protein homotetramerization
G	0070062	cellular_component	extracellular exosome
G	0070814	biological_process	hydrogen sulfide biosynthetic process
G	0080146	molecular_function	L-cysteine desulfhydrase activity
G	0098606	molecular_function	selenocystathionine gamma-lyase activity
G	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
G	1990830	biological_process	cellular response to leukemia inhibitory factor
G	2001234	biological_process	negative regulation of apoptotic signaling pathway
H	0004123	molecular_function	cystathionine gamma-lyase activity
H	0005515	molecular_function	protein binding
H	0005516	molecular_function	calmodulin binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006534	biological_process	cysteine metabolic process
H	0006629	biological_process	lipid metabolic process
H	0016829	molecular_function	lyase activity
H	0018272	biological_process	protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine
H	0019343	biological_process	cysteine biosynthetic process via cystathionine
H	0019344	biological_process	cysteine biosynthetic process
H	0019346	biological_process	transsulfuration
H	0030170	molecular_function	pyridoxal phosphate binding
H	0030968	biological_process	endoplasmic reticulum unfolded protein response
H	0042802	molecular_function	identical protein binding
H	0043066	biological_process	negative regulation of apoptotic process
H	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
H	0044524	biological_process	protein sulfhydration
H	0044540	molecular_function	L-cystine L-cysteine-lyase (deaminating)
H	0047982	molecular_function	homocysteine desulfhydrase activity
H	0051289	biological_process	protein homotetramerization
H	0070062	cellular_component	extracellular exosome
H	0070814	biological_process	hydrogen sulfide biosynthetic process
H	0080146	molecular_function	L-cysteine desulfhydrase activity
H	0098606	molecular_function	selenocystathionine gamma-lyase activity
H	1904831	biological_process	positive regulation of aortic smooth muscle cell differentiation
H	1990830	biological_process	cellular response to leukemia inhibitory factor
H	2001234	biological_process	negative regulation of apoptotic signaling pathway

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SO4 A 501

Chain	Residue
A	TYR114
A	ASN161
A	LLP212
A	VAL339
A	SER340
A	LEU341
A	THR355
A	ARG375

site_id	AC2
Number of Residues	7
Details	binding site for residue SO4 C 501

Chain	Residue
C	LLP212
C	VAL339
C	SER340
C	LEU341
C	THR355
C	ARG375
C	TYR114

Functional Information from PROSITE/UniProt

site_id	PS00868
Number of Residues	15
Details	CYS_MET_METAB_PP Cys/Met metabolism enzymes pyridoxal-phosphate attachment site. DIsmySATKYMnGHS

Chain	Residue	Details
A	ASP204-SER218

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	32
Details	BINDING:

Chain	Residue	Details
A	ARG62
C	TYR114
C	ALA119
C	VAL339
D	ARG62
D	TYR114
D	ALA119
D	VAL339
E	ARG62
E	TYR114
E	ALA119
A	TYR114
E	VAL339
F	ARG62
F	TYR114
F	ALA119
F	VAL339
G	ARG62
G	TYR114
G	ALA119
G	VAL339
H	ARG62
A	ALA119
H	TYR114
H	ALA119
H	VAL339
A	VAL339
B	ARG62
B	TYR114
B	ALA119
B	VAL339
C	ARG62

site_id	SWS_FT_FI2
Number of Residues	8
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269\|PubMed:19019829

Chain	Residue	Details
A	LLP212
B	LLP212
C	LLP212
D	LLP212
E	LLP212
F	LLP212
G	LLP212
H	LLP212

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)