6NYO
Crystal structure of a human Cdc34-ubiquitin thioester mimetic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 2017-02-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.585, 55.737, 119.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.784 - 1.502 |
R-factor | 0.1759 |
Rwork | 0.175 |
R-free | 0.20220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mdk |
RMSD bond length | 0.006 |
RMSD bond angle | 0.786 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.069 | 0.976 |
Rpim | 0.032 | 0.464 |
Number of reflections | 48439 | |
<I/σ(I)> | 17.5 | |
Completeness [%] | 99.9 | |
Redundancy | 5.67 | |
CC(1/2) | 0.997 | 0.582 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.03 M potassium dihydrogen phosphate, 23% PEG 8,000 |