6MAC

Ternary structure of GDF11 bound to ActRIIB-ECD and Alk5-ECD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033202
Spacegroup name	P 32 1 2
Unit cell lengths	116.624, 116.624, 56.625
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.500 - 2.340
R-factor	0.2261
Rwork	0.222
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e4g 3kfd 1nys
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.500	2.424
High resolution limit [Å]	2.340	2.340
Rmerge	0.027	0.402
Rmeas	0.038	0.569
Rpim	0.027	0.402
Number of reflections	18669	1861
<I/σ(I)>	12.07	1.51
Completeness [%]	99.9	99.95
Redundancy	2	2
CC(1/2)	0.999	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	.05-.15M sodium acetate 12-24% polyethylene glycol 8000 .05-.15M sodium thiocyanate