6M8S

Crystal structure of the KCTD12 H1 domain in complex with Gbeta1gamma2 subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	109.090, 121.990, 206.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.406 - 3.710
R-factor	0.2533
Rwork	0.251
R-free	0.28660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1omw
RMSD bond length	0.003
RMSD bond angle	0.706
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.800
High resolution limit [Å]	3.710	3.710
Rmerge		1.059
Rmeas		1.213
Number of reflections	29623	2129
<I/σ(I)>	3.56	1.09
Completeness [%]	99.0	97.6
Redundancy	3.9	3.9
CC(1/2)		0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium chloride, 0.1 M sodium cacodylate, 8% w/v PEG8000