6KA6
Crystal structure of plasmodium lysyl-tRNA synthetase in complex with a cladosporin derivative 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-04-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 |
Unit cell lengths | 71.479, 90.202, 100.720 |
Unit cell angles | 76.70, 72.91, 80.03 |
Refinement procedure
Resolution | 32.597 - 1.891 |
R-factor | 0.1931 |
Rwork | 0.191 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ycv |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.680 | 38.680 | 1.940 |
High resolution limit [Å] | 1.890 | 8.450 | 1.890 |
Rmerge | 0.122 | 0.130 | 0.825 |
Number of reflections | 164813 | 1874 | 7979 |
<I/σ(I)> | 5 | ||
Completeness [%] | 88.8 | 89.7 | 57.8 |
Redundancy | 3.5 | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | tri-sodium citrate, PEG 3350 |