6I01
Structure of human D-glucuronyl C5 epimerase in complex with substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97692 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.675, 99.675, 258.823 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.320 - 2.100 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hzz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 1.550 |
| Number of reflections | 80072 | |
| <I/σ(I)> | 12.6 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M Na acetate pH 5.5, 24% MPEG 5K and 0.1 M MES pH 6.5, 1.25 M lithium acetate |
