6I01
Structure of human D-glucuronyl C5 epimerase in complex with substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | D-glucuronyl C5-epimerase | polymer | 527 | 60294.4 | 2 | UniProt (O94923) Pfam (PF21174) Pfam (PF06662) | Homo sapiens (Human) | Heparan sulfate C5-epimerase,Hsepi,Heparin/heparan sulfate:glucuronic acid C5-epimerase,Heparosan-N-sulfate-glucuronate 5-epimerase |
| 2 | C, F (C, F) | beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid | branched | 1446.2 | 2 | In PDB GlyTouCan (G92964EB) | |||
| 3 | D (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1114.0 | 1 | In PDB GlyTouCan (G61751GZ) | |||
| 4 | E, H (E, H) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 5 | G (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 951.9 | 1 | In PDB GlyTouCan (G12045WP) | |||
| 6 | I (I) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 7 | J (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 8 | K, L, M, N, O... (A, B) | GLYCEROL | non-polymer | 92.1 | 14 | Chemie (GOL) | |||
| 9 | R (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 10 | AA, S (B, A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 11 | BA, CA (A, B) | water | water | 18.0 | 564 | Chemie (HOH) |
Sequence modifications
A, B: 98 - 617 (UniProt: O94923)
| PDB | External Database | Details |
|---|---|---|
| Asp 91 | - | expression tag |
| Tyr 92 | - | expression tag |
| Lys 93 | - | expression tag |
| Asp 94 | - | expression tag |
| Asp 95 | - | expression tag |
| Asp 96 | - | expression tag |
| Asp 97 | - | expression tag |
| Phe 578 | Tyr 578 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 120588.7 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 7204.3 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 1649.7 | |
| All* | Total formula weight | 129442.7 |
