6HYC
The structure of full-length human phenylalanine hydroxylase in complex with the cofactor and negative regulator tetrahydrobiopterin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.940, 101.368, 203.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.920 - 3.180 |
| R-factor | 0.2641 |
| Rwork | 0.262 |
| R-free | 0.31070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5den |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.920 | 3.340 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.181 | |
| Rpim | 0.131 | |
| Number of reflections | 34280 | 3926 |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 94.3 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1.5 M DL Malic Acid pH=7.0, 100 mM Bis-Tris Propane pH=6.2, 0.9 mM Thesit, 0.98 mM BH4, 25 mM DTT and 1 mM reduced glutathione |
