6FYH

Disulfide between ubiquitin G76C and the E3 HECT ligase Huwe1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.002
Spacegroup name	P 43 21 2
Unit cell lengths	82.234, 82.234, 85.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.144 - 2.906
R-factor	0.2316
Rwork	0.230
R-free	0.26560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h1d 4bbn
RMSD bond length	0.002
RMSD bond angle	0.455
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.144	3.080
High resolution limit [Å]	2.906	2.910
Rmerge	0.126	0.853
Number of reflections	12356
<I/σ(I)>	21.43
Completeness [%]	99.6
Redundancy	16.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8M Zn SO4 0.1M Na Acetat pH 4.0