6FYH

Disulfide between ubiquitin G76C and the E3 HECT ligase Huwe1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 ubiquitin-protein ligase HUWE1	polymer	124	14198.9	1	UniProt (Q7Z6Z7) Pfam (PF00632) In PDB	Homo sapiens (Human)	ARF-binding protein 1,ARF-BP1,HECT,UBA and WWE domain-containing protein 1,HECT-type E3 ubiquitin transferase HUWE1,Homologous to E6AP carboxyl terminus homologous protein 9,HectH9,Large structure of UREB1,LASU1,Mcl-1 ubiquitin ligase E3,Mule,Upstream regulatory element-binding protein 1,URE-binding protein 1
2	B	Polyubiquitin-B	polymer	76	8622.9	1	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
3	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	A, B	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
5		water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 266 - 385 (UniProt: Q7Z6Z7)

PDB	External Database	Details
Gly 262	-	expression tag
Ala 263	-	expression tag
Met 264	-	expression tag
Gly 265	-	expression tag
Asn 378	Cys 4367	engineered mutation

B: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	22821.9
Non-Polymers*	Number of molecules	8
	Total formula weight	584.6
All*	Total formula weight	23406.4

*Water molecules are not included.