6FYH

Disulfide between ubiquitin G76C and the E3 HECT ligase Huwe1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004842	molecular_function	ubiquitin-protein transferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SO4 A 401

Chain	Residue
A	ILE268
A	ILE268
A	ILE270
A	ILE270
A	ARG297
A	ARG297
A	ARG304
A	ARG304

---

site_id	AC2
Number of Residues	3
Details	binding site for residue ZN A 402

Chain	Residue
A	HIS369
A	HOH503
A	GLU365

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 403

Chain	Residue
A	HIS280
A	HIS337
A	GLU365
A	ZN406

---

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN A 404

Chain	Residue
A	HIS350
A	ASP357
A	SO4405
A	HOH502

---

site_id	AC5
Number of Residues	7
Details	binding site for residue SO4 A 405

Chain	Residue
A	ARG341
A	HIS350
A	ASP357
A	GLU362
A	LYS366
A	ZN404
B	ZN101

---

site_id	AC6
Number of Residues	5
Details	binding site for residue ZN A 406

Chain	Residue
A	HIS280
A	HIS337
A	GLU365
A	ZN403
A	HOH505

---

site_id	AC7
Number of Residues	1
Details	binding site for residue ZN B 101

Chain	Residue
A	SO4405

---

site_id	AC8
Number of Residues	1
Details	binding site for residue ZN B 102

Chain	Residue
B	ASP32

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
B	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
B	ARG54
B	ARG72

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: Essential for function

Chain	Residue	Details
B	HIS68

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
B	SER65

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
B	THR66

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269|PubMed:28525742

Chain	Residue	Details
B	CYS76

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
B	LYS6

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
B	CYS76

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
B	LYS11
B	LYS48

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
B	LYS27

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
B	LYS29

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
B	LYS33

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
B	LYS63

---