6F57

Crystal structure of DNMT3A-DNMT3L in complex with single CpG-containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 21 21 2
Unit cell lengths	176.611, 49.559, 162.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.720 - 3.098
R-factor	0.2133
Rwork	0.211
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qrv
RMSD bond length	0.005
RMSD bond angle	0.776
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	3.200
High resolution limit [Å]	3.090	3.090
Rmerge	0.176	0.982
Number of reflections	26661
<I/σ(I)>	9.1
Completeness [%]	99.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	7% PEG4000, 0.1 M Tris-HCl (pH 8.5), 100 mM MgCl2, 166 mM imidazole (pH 7.0)