6ERF
Complex of APLF factor and Ku heterodimer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 98.920, 140.860, 150.310 |
| Unit cell angles | 68.64, 80.85, 81.23 |
Refinement procedure
| Resolution | 49.470 - 3.010 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jey |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 3.280 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Rmerge | 0.056 | 0.790 |
| Rmeas | 0.072 | 0.930 |
| Rpim | 0.051 | 0.660 |
| Number of reflections | 90993 | |
| <I/σ(I)> | 11.8 | 1.3 |
| Completeness [%] | 61.9 | 14.3 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM Bris Tris propane 13% PEG 3350 150 mM Na nirate |
