6E2H
Crystal structure of human Ash2L (SPRY domain and SDI motif) in complex with full length DPY-30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 103.011, 103.011, 129.138 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.443 - 2.236 |
R-factor | 0.178 |
Rwork | 0.177 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4riq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.679 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.443 | 2.310 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 33958 | 402 |
<I/σ(I)> | 74.1 | |
Completeness [%] | 99.4 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 20mM citrate pH 4.7 and polyethylene glycol (PEG) 6000 |