6E2H

Crystal structure of human Ash2L (SPRY domain and SDI motif) in complex with full length DPY-30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	103.011, 103.011, 129.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.443 - 2.236
R-factor	0.178
Rwork	0.177
R-free	0.19970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4riq
RMSD bond length	0.003
RMSD bond angle	0.679
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.443	2.310
High resolution limit [Å]	2.200	2.200
Number of reflections	33958	402
<I/σ(I)>	74.1
Completeness [%]	99.4
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20mM citrate pH 4.7 and polyethylene glycol (PEG) 6000