6D94
Crystal structure of PPAR gamma in complex with Mediator of RNA polymerase II transcription subunit 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-03 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.437, 87.623, 121.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.812 - 1.900 |
| R-factor | 0.2031 |
| Rwork | 0.199 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tto |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13rc2_2975: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.810 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.090 | 0.560 |
| Number of reflections | 23571 | 2313 |
| <I/σ(I)> | 19.2 | 7.6 |
| Completeness [%] | 99.3 | 98.72 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100mM HEPES 25% (w/v) PEG 2000 MME |
