6B0G
Crystal structure of Pfs25 in complex with the transmission blocking antibody 1245
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.772, 83.692, 76.212 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 38.989 - 1.900 |
| R-factor | 0.1653 |
| Rwork | 0.164 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d1x 1z27 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | XPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.065 | 0.351 |
| Number of reflections | 51093 | 7230 |
| <I/σ(I)> | 11.8 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 294 | 0.1 M citric acid pH 5.0, 1 M lithium chloride, 20 % (w/v) PEG 6000 |
