6UE5

Crystal structure of full-length human DCAF15-DDB1-deltaPBP-DDA1-RBM39 in complex with 4-(aminomethyl)-N-(3-cyano-4-methyl-1H-indol-7-yl)benzenesulfonamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-17
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	81.692, 94.237, 267.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.130 - 2.610
R-factor	0.241
Rwork	0.239
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ud7
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.790	2.690
High resolution limit [Å]	2.610	2.610
Rmerge	0.487	3.221
Rmeas	0.500	3.309
Rpim	0.113	0.752
Number of reflections	63346	4597
<I/σ(I)>	6.2
Completeness [%]	100.0	100
Redundancy	19.6	19.3
CC(1/2)	0.985	0.326

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.5	2% (v:v) TacsimateTM, pH 5.0, 0.1 M sodium citrate tribasic 676 dihydrate, pH 5.6, and 10-20% (w:v) polyethylene glycol 3350