6SS6

Structure of arginase-2 in complex with the inhibitory human antigen-binding fragment Fab C0020187

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 65 2 2
Unit cell lengths	138.126, 138.126, 551.307
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.755 - 3.250
Rwork	0.300
R-free	0.36060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hze 6ss5
RMSD bond length	0.005
RMSD bond angle	1.463
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	3.360
High resolution limit [Å]	3.250	3.250
Rmerge	0.780	8.480
Number of reflections	50453	4555
<I/σ(I)>	4.2	0.4
Completeness [%]	100.0	100
Redundancy	10.4	9.9
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2 M (NH4)2SO4