6OVG
L-Methionine Depletion with an Engineered Human Enzyme Disrupts Prostate Cancer Metabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 113.558, 164.330, 181.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.337 - 2.719 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nmp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.719 | 2.719 |
| Number of reflections | 175177 | 5110 |
| <I/σ(I)> | 13.109 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% isopropanol, 150mM sodium citrate 100mM sodium cocadylate pH 6.5 |
