6F57
Crystal structure of DNMT3A-DNMT3L in complex with single CpG-containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 176.611, 49.559, 162.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.720 - 3.098 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qrv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.776 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 3.200 |
High resolution limit [Å] | 3.090 | 3.090 |
Rmerge | 0.176 | 0.982 |
Number of reflections | 26661 | |
<I/σ(I)> | 9.1 | |
Completeness [%] | 99.3 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 7% PEG4000, 0.1 M Tris-HCl (pH 8.5), 100 mM MgCl2, 166 mM imidazole (pH 7.0) |