6ERF

Complex of APLF factor and Ku heterodimer bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 1
Unit cell lengths	98.920, 140.860, 150.310
Unit cell angles	68.64, 80.85, 81.23

Refinement procedure

Resolution	49.470 - 3.010
R-factor	0.21
Rwork	0.209
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jey
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.470	3.280
High resolution limit [Å]	3.010	3.010
Rmerge	0.056	0.790
Rmeas	0.072	0.930
Rpim	0.051	0.660
Number of reflections	90993
<I/σ(I)>	11.8	1.3
Completeness [%]	61.9	14.3
Redundancy	3.4	3.5
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100 mM Bris Tris propane 13% PEG 3350 150 mM Na nirate