5ZMD
Crystal structure of FTO in complex with m6dA modified ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2017-04-16 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9765 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 122.736, 160.033, 276.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.170 - 3.300 |
| R-factor | 0.272 |
| Rwork | 0.271 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lfm |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.097 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.292 | 2.017 |
| Rpim | 0.811 | |
| Number of reflections | 41219 | |
| <I/σ(I)> | 7.9 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.852 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | 20% PEG 3350, pH 7.0 |
