5Y3M
Crystal structure of bovine TLR9 in complex with two DNAs (CpG DNA and TCGTTT DNA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 88.177, 68.693, 153.535 |
| Unit cell angles | 90.00, 100.64, 90.00 |
Refinement procedure
| Resolution | 48.960 - 2.500 |
| R-factor | 0.22606 |
| Rwork | 0.225 |
| R-free | 0.24427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wpe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.960 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 62911 |
| <I/σ(I)> | 8 |
| Completeness [%] | 99.9 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10%(w/v) PEG 4000 0.5M NaCl 0.1M Mes-NaOH pH 6.5 |
