5OMW
Mutant T252A of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucyl-adenylate analogue in the aminoacylation conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.87260 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.250, 68.910, 228.330 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 220.620 - 2.600 |
| R-factor | 0.22092 |
| Rwork | 0.219 |
| R-free | 0.26416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aq7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.131 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 220.620 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 73538 | |
| <I/σ(I)> | 7.8 | 1.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.5 | 3.69 |
| CC(1/2) | 0.992 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M BIS-TRIS PH 5.5, 23-25% W/V PEG 3350, AND 200 MM AMMONIUM ACETATE |
