5OMW
Mutant T252A of E. coli leucyl-tRNA synthetase, tRNA(leu) and leucyl-adenylate analogue in the aminoacylation conformation
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | Leucine--tRNA ligase | polymer | 880 | 99486.0 | 2 | UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264) In PDB | Escherichia coli K-12 | Leucyl-tRNA synthetase,LeuRS |
2 | B, E | L-leucyl-tRNA | polymer | 87 | 28052.7 | 2 | GenBank (1207385735) | Escherichia coli | |
3 | A, D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
4 | A, D | 5'-O-(L-leucylsulfamoyl)adenosine | non-polymer | 459.5 | 2 | Chemie (LSS) | |||
5 | B, E | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) |
Sequence modifications
A, D: 1 - 860 (UniProt: P07813)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 252 | Thr 252 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 255077.3 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1098.4 | |
All* | Total formula weight | 256175.7 |