5F9F
Crystal structure of RIG-I helicase-RD in complex with 24-mer blunt-end hairpin RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.524, 174.252, 308.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.994 - 2.601 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e3h |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.579 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.601 | 2.600 |
| Number of reflections | 166166 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 90.6 | 80.3 |
| Redundancy | 13 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% (w/v) PEG 3350, 0.3 M NaSCN, 100 mM MOPS (pH 7.8) |
