5C1M
Crystal structure of active mu-opioid receptor bound to the agonist BU72
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2014-11-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 44.430, 144.000, 209.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.470 | 2.120 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.112 | 1.536 |
Number of reflections | 41704 | 2793 |
<I/σ(I)> | 11 | 1.7 |
Completeness [%] | 99.8 | |
Redundancy | 8.6 | |
CC(1/2) | 0.992 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.5 | 293 | Reconstituted in 10:1 monoolein:cholesterol mix. Precipitant solution:15-25% PEG300, 100 mM HEPES pH 7.0-7.5, 1% 1,2,3-heptanetriol, 0.5-1.0% Polypropylene glycol P 400, 100-300 mM (NH4)2HPO4 |