5ZMD

Crystal structure of FTO in complex with m6dA modified ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	180
Detector technology	CCD
Collection date	2017-04-16
Detector	BRUKER SMART 6500
Wavelength(s)	0.9765
Spacegroup name	I 21 21 21
Unit cell lengths	122.736, 160.033, 276.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.170 - 3.300
R-factor	0.272
Rwork	0.271
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lfm
RMSD bond length	0.021
RMSD bond angle	2.097
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.292	2.017
Rpim		0.811
Number of reflections	41219
<I/σ(I)>	7.9	1.1
Completeness [%]	99.9	100
Redundancy	7	7.1
CC(1/2)		0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	273	20% PEG 3350, pH 7.0