5F9F
Crystal structure of RIG-I helicase-RD in complex with 24-mer blunt-end hairpin RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-18 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.524, 174.252, 308.288 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.994 - 2.601 |
R-factor | 0.2056 |
Rwork | 0.203 |
R-free | 0.25940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5e3h |
RMSD bond length | 0.002 |
RMSD bond angle | 0.579 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.601 | 2.600 |
Number of reflections | 166166 | |
<I/σ(I)> | 13 | 2.4 |
Completeness [%] | 90.6 | 80.3 |
Redundancy | 13 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% (w/v) PEG 3350, 0.3 M NaSCN, 100 mM MOPS (pH 7.8) |