4ZCS
Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with CDP-choline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979338 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 114.631, 149.132, 175.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.670 - 2.450 |
| R-factor | 0.18325 |
| Rwork | 0.181 |
| R-free | 0.23413 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hl4 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 113.670 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 52521 |
| <I/σ(I)> | 0.2 |
| Completeness [%] | 99.6 |
| Redundancy | 9.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.2M NaF, 20% PEG 3350 |
