4XMN
Structure of the yeast coat nucleoporin complex, space group P212121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 117.130, 179.950, 441.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.606 - 7.600 |
R-factor | 0.3212 |
Rwork | 0.318 |
R-free | 0.34730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iko 3f7f 3pgf |
RMSD bond length | 0.005 |
RMSD bond angle | 1.123 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1809)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 7.600 |
Number of reflections | 22082 |
<I/σ(I)> | 13.5 |
Completeness [%] | 99.2 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | PEG 20000, ethanol, MES |