4XMN

Structure of the yeast coat nucleoporin complex, space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	117.130, 179.950, 441.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.606 - 7.600
R-factor	0.3212
Rwork	0.318
R-free	0.34730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iko 3f7f 3pgf
RMSD bond length	0.005
RMSD bond angle	1.123
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1809))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	7.600
Number of reflections	22082
<I/σ(I)>	13.5
Completeness [%]	99.2
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	PEG 20000, ethanol, MES