4XMN

Structure of the yeast coat nucleoporin complex, space group P212121

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein transport protein SEC13	polymer	297	33129.9	1	UniProt (Q04491) Pfam (PF00400)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B (B)	Nucleoporin NUP145	polymer	652	75087.7	1	UniProt (P49687) Pfam (PF12110)	Saccharomyces cerevisiae (Baker's yeast)	Nuclear pore protein NUP145
3	C (F)	Nucleoporin NUP84	polymer	454	52435.0	1	UniProt (P52891) Pfam (PF04121)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Nuclear pore protein NUP84
4	D (E)	Nucleoporin NUP120	polymer	1045	121607.5	1	UniProt (P35729) Pfam (PF22114)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Nuclear pore protein NUP120
5	E (D)	Nucleoporin NUP85	polymer	685	79188.0	1	UniProt (P46673) Pfam (PF07575)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Nuclear pore protein NUP85
6	F (L)	Antibody 87 light chain	polymer	217	23461.1	1		synthetic construct
7	G (H)	Antibody 87 heavy chain	polymer	267	28615.9	1		synthetic construct

Sequence modifications

A: 2 - 297 (UniProt: Q04491)

PDB	External Database	Details
Mse 1	-	initiating methionine

B: 75 - 712 (UniProt: P49687)

PDB	External Database	Details
Mse 61	-	initiating methionine
Gly 62	-	expression tag
Ser 63	-	expression tag
Ser 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
His 70	-	expression tag
Ser 71	-	expression tag
Asp 72	-	expression tag
Gln 73	-	expression tag
Pro 74	-	expression tag

F: 2 - 451 (UniProt: P52891)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Mse 1	-	expression tag

E: 1 - 1037 (UniProt: P35729)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag

D: 73 - 743 (UniProt: P46673)

PDB	External Database	Details
Mse 60	-	initiating methionine
Gly 61	-	expression tag
Ser 62	-	expression tag
Ser 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag
His 67	-	expression tag
His 68	-	expression tag
His 69	-	expression tag
Ser 70	-	expression tag
Asp 71	-	expression tag
Gln 72	-	expression tag
Mse 744	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	413525.0
All*	Total formula weight	413525.0

*Water molecules are not included.