4RHU
Crystal structures of Mycobacterium tuberculosis 6-oxopurine phosphoribosyltransferase which is a potential target for drug development against this disease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.691, 94.691, 335.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.346 - 2.573 |
| R-factor | 0.2329 |
| Rwork | 0.230 |
| R-free | 0.29830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rhx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.607 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.350 | 2.650 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.772 | |
| Number of reflections | 53590 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 100.0 | 98.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M potassium sodium tartrate, 0.1 M imidazole pH 8.0, 0.7 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
