4OGR
crystal structure of P-TEFb complex with AFF4 and Tat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.11 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 184.908, 184.908, 360.399 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.015 - 3.000 |
R-factor | 0.2056 |
Rwork | 0.205 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4imy |
RMSD bond length | 0.004 |
RMSD bond angle | 0.805 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1419) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
Rmerge | 0.222 | 0.044 | |
Number of reflections | 73424 | ||
<I/σ(I)> | 12 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 24.2 | 22.4 | 23.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | Crystallized from 25 mM HEPES pH 7.3, 0.2 M NaCl, 10 mM MgCl, 2.4 M Na formate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |